Oportunidades de bolsas de pós-graduação

  O Grupo de Biotecnologia Molecular do IFSC/USP procura candidatos para bolsas de Doutorado Direto, Doutorado e Mestrado, interessados em desenvolver trabalhos nas áreas de Biologia Estrutural, Biotecnologia Molecular e Biofísica. Há oportunidades em dois projetos do grupo. O primeiro projeto visa compreender as bases moleculares da hidrólise enzimática de polissacarídeos de parede celular de […]

High-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC).

Recent advances in DNA sequencing techniques have led to an explosion in the amount of available genome sequencing data and this provided an inexhaustible source of uncharacterized glycoside hydrolases (GH) to be studied both structurally and enzymatically. Ligation-Independent Cloning (LIC), an interesting alternative to traditional, restriction enzyme-based cloning, and commercial recombinatorial cloning, was adopted and […]

Family 1 carbohydrate binding-modules enhance saccharification rates.

Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolysis activity enhancement promoted by the cellobiohydrolase I carbohydrate-binding module from […]

Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome.

In recent years, owing to the growing global demand for energy, dependence on fossil fuels, limited natural resources and environmental pollution, biofuels have attracted great interest as a source of renewable energy. However, the production of biofuels from plant biomass is still considered to be an expensive technology. In this context, the study of carbohydrate-binding […]

Bumblebees sense electric fields in flowers

  As they zero in on their sugary reward, foraging bumblebees follow an invisible clue: electric fields. Although some animals, including sharks, are known to have an electric sense, this is the first time the ability has been documented in insects.   Read the full history in http://www.nature.com/news/bumblebees-sense-electric-fields-in-flowers-1.12480