Following burgeoning genomic and transcriptomic sequencing data, biochemical and molecular biology groups worldwide are implementing high-throughput cloning and mutagenesis facilities in order to obtain a large number of soluble proteins for structural and functional characterization. Since manual primer design can be a time-consuming and error-generating step, particularly when working with hundreds of targets, the automation […]
Programming: Pick up Python
A powerful programming language with huge community support. Article in Nature. Nature 518, 125–126 (05 February 2015) doi:10.1038/518125a
Nuclear receptor full-length architectures: confronting myth and illusion with high resolution
The crystal structures of three nuclear receptor (NR) complexes have emerged to reveal their multidomain architectures on DNA. These pictures provide unprecedented views of interfacial couplings between the DNA-binding domains (DBDs) and ligand-binding domains (LBDs). The detailed pictures contrast with previous interpretations of low-resolution electron microscopy (EM) and small angle X-ray scattering (SAXS) data, which […]
RXR agonist modulates TR: corepressor dissociation upon 9-cis retinoic acid treatment
Transcriptional regulation controlled by thyroid hormone receptor (TR) drives events such as development, differentiation and metabolism. TRs may act either as homodimers, or as heterodimers with retinoid X receptor (RXR). Thyroid hormone T3 (3, 3`,5 triiodo-L-thyronine) preferentially binds TR:RXR heterodimers, which activate transcription through coactivator recruitment. However, it is unclear if TR:RXR heterodimers may also […]