Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme

Trichoderma filamentous fungi have been investigated due to their ability to secrete cellulases which find various biotechnological applications such as biomass hydrolysis and cellulosic ethanol production. Previous studies demonstrated that Trichoderma harzianum IOC-3844 has a high degree of cellulolytic activity and potential for biomass hydrolysis. However, enzymatic, biochemical, and structural studies of cellulases from T. […]

Agência FAPESP – Aprimorar coquetéis enzimáticos é caminho para etanol celulósico

15/05/2012 Por Fábio de Castro Agência FAPESP – Novos avanços científicos tornaram a produção de etanol celulósico realidade, mas ainda é preciso um esforço concentrado de pesquisa para torná-la economicamente viável em escala industrial. O caminho mais curto para cumprir esse objetivo é a intensificação dos estudos que visam aperfeiçoar e otimizar os coquetéis de enzimas […]

X-ray Structure and Molecular Dynamics Simulations of Endoglucanase 3 from Trichoderma harzianum: Structural Organization and Substrate Recognition by Endoglucanases That Lack Cellulose Binding Module

X-ray Structure and Molecular Dynamics Simulations of Endoglucanase 3 from Trichoderma harzianum: Structural Organization and Substrate Recognition by Endoglucanases That Lack Cellulose Binding Module Érica T. Prates, Ivana Stankovic, Rodrigo L. Silveira, Marcelo V. Liberato, Flávio Henrique-Silva, Nei Pereira Jr, Igor Polikarpov, Munir S. Skaf   Plant biomass holds a promise for the production of […]

Joint x-ray crystallographic and molecular dynamics study of cellobiohydrolase I from trichoderma harzianum: deciphering the structural features of cellobiohydrolase catalytic activity

Textor LC, Colussi F, Silveira RL, Serpa V, de Mello BL, Muniz JR, Squina FM, Pereira N Jr, Skaf MS, Polikarpov I.   Aiming to contribute towards the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of Cel7A (CBHI) from the filamentous fungus Trichoderma harzianum IOC 3844. Our structural studies and molecular dynamics […]