{"id":9670,"date":"2022-06-08T15:31:21","date_gmt":"2022-06-08T18:31:21","guid":{"rendered":"https:\/\/www.ifsc.usp.br\/multiusuarios\/?page_id=9670"},"modified":"2026-03-24T15:45:02","modified_gmt":"2026-03-24T18:45:02","slug":"lbest-mst","status":"publish","type":"page","link":"https:\/\/www.ifsc.usp.br\/multiusuarios\/lbest-mst\/","title":{"rendered":"LBEst &#8211; MicroScale Thermophoresis (MST)"},"content":{"rendered":"<p>[vc_row rt_row_background_width=&#8221;default&#8221; rt_row_style=&#8221;default-style&#8221; rt_row_borders=&#8221;&#8221; rt_row_paddings=&#8221;true&#8221; rt_bg_effect=&#8221;classic&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;cover&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_bg_video_format=&#8221;self-hosted&#8221;][vc_column rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_padding_left=&#8221;60px&#8221; rt_padding_right=&#8221;60px&#8221;][vc_column_text]<\/p>\n<h4><strong>MicroScale Thermophoresis (MST)<\/strong><\/h4>\n<p><strong>Laborat\u00f3rio de Bioqu\u00edmica no Grupo de Biof\u00edsica e Biologia Estrutural &#8211; IFSC &#8211; USP &#8211; \u00c1rea 2<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-9671 alignright\" src=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst1-300x218.png\" alt=\"\" width=\"300\" height=\"218\" srcset=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst1-300x218.png 300w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst1-768x559.png 768w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst1.png 800w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>Comit\u00ea Gestor:<\/strong><\/p>\n<ul>\n<li>Prof. Dr. Glaucius Oliva<\/li>\n<li>Profa. Dra. Leila Maria Beltramini<\/li>\n<li>Prof. Dr. Rafael V. C. Guido<\/li>\n<\/ul>\n<p>E-mail para contato: oliva@ifsc.usp.br[\/vc_column_text][\/vc_column][\/vc_row][vc_row rt_row_background_width=&#8221;default&#8221; rt_row_style=&#8221;default-style&#8221; rt_row_borders=&#8221;&#8221; rt_row_paddings=&#8221;true&#8221; rt_bg_effect=&#8221;classic&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;cover&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_bg_video_format=&#8221;self-hosted&#8221;][vc_column width=&#8221;1\/2&#8243; rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_padding_left=&#8221;60px&#8221;][vc_message icon_fontawesome=&#8221;fa fa-cog&#8221;]<strong>Equipamento<\/strong><\/p>\n<p>O equipamento NanoTemper Monolith NT.115 utiliza o princ\u00edpio da termoforese em microescala (MST) para identifica\u00e7\u00e3o e quantifica\u00e7\u00e3o de intera\u00e7\u00f5es biomoleculares. Basicamente, o equipamento mede a movimenta\u00e7\u00e3o de mol\u00e9culas ao longo de gradientes microsc\u00f3picos de temperatura e detecta mudan\u00e7as em suas camadas de hidrata\u00e7\u00e3o, carga e tamanho. Dessa forma ele se torna uma ferramenta extremamente vers\u00e1til, oferendo medidas precisas de intera\u00e7\u00e3o (Kd) numa ampla faixa de afinidades (pM at\u00e9 mM) e massas moleculares (desde \u00edons at\u00e9 grandes complexos prot\u00e9icos). \u00c9 poss\u00edvel obter medidas de afinidades de prote\u00edna-prote\u00edna, prote\u00edna-pequena mol\u00e9cula, prote\u00edna-DNA\/RNA, por exemplo.[\/vc_message][vc_message]<strong>Miss\u00e3o<\/strong><\/p>\n<p>Disponibilizar um equipamento capaz de quantificar de intera\u00e7\u00f5es biomoleculares para qualquer pesquisador interessado em obter medidas de afinidades entre prote\u00edna-prote\u00edna, prote\u00edna-pequena mol\u00e9cula e prote\u00edna-DNA\/RNA.<\/p>\n<p><strong>Caracter\u00edsticas<\/strong><\/p>\n<p>Determina\u00e7\u00e3o precisa de intera\u00e7\u00e3o (Kd) numa ampla faixa de afinidades (pM at\u00e9 mM) e massas moleculares (desde \u00edons at\u00e9 grandes complexos prot\u00e9icos).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-9672 size-medium\" src=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-300x222.png\" alt=\"\" width=\"300\" height=\"222\" srcset=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-300x222.png 300w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-1024x756.png 1024w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-768x567.png 768w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-1536x1135.png 1536w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/mst-caract-2048x1513.png 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>[\/vc_message][\/vc_column][vc_column width=&#8221;1\/2&#8243; rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_padding_right=&#8221;60px&#8221;][vc_message icon_fontawesome=&#8221;fa fa-user-o&#8221;]<strong>Plano de Gest\u00e3o e Uso:<\/strong><\/p>\n<p>\u2013 O equipamento MicroScale Thermophoresis (MST) ser\u00e1 instalado no Laborat\u00f3rio de Bioqu\u00edmica no Grupo de Biof\u00edsica Molecular.<\/p>\n<p>\u2013 As despesas com consum\u00edveis ser\u00e3o custeadas pelo usu\u00e1rio, o custo aproximado \u00e9 de US$ 5,00 por capilar, para utiliza\u00e7\u00e3o do MicroScale Thermophoresis.<\/p>\n<p>\u2013 Usu\u00e1rios que tenham interesse em utilizar os equipamentos, sejam do IFSC ou de qualquer outra institui\u00e7\u00e3o, dever\u00e3o submeter ao comit\u00ea gestor, via e-mail gryphon_mals@ifsc.usp.br, formul\u00e1rio obtido no seguinte link: <a href=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/formulario-lbest-mst.docx\">Formul\u00e1rio Proposta de Uso<\/a>. Neste formul\u00e1rio dever\u00e3o ser fornecidas as caracter\u00edsticas das amostras (peso molecular, concentra\u00e7\u00e3o, pureza, estado de oligomeriza\u00e7\u00e3o, tamp\u00e3o, Gel de SDS da purifica\u00e7\u00e3o, concentra\u00e7\u00e3o de sal e outros aditivos etc.) dentro de um plano de trabalho conciso contendo os principais objetivos do projeto. Um equipamento de <em>Dynamic Light Scatering<\/em> (DLS) est\u00e1 dispon\u00edvel para uma caracteriza\u00e7\u00e3o da amostra. Sugerimos fortemente uma consulta previa para que seja fornecido um aux\u00edlio para a sugest\u00e3o de tamp\u00f5es adequados para a prepara\u00e7\u00e3o da amostra. O pedido ser\u00e1 analisado pelo comit\u00ea gestor, que pode negar o acesso caso haja problemas com a amostra da prote\u00edna.<\/p>\n<p>\u2013 O agendamento ser\u00e1 mensal, priorizando-se os Projetos Associados e Complementares desta solicita\u00e7\u00e3o.<\/p>\n<p>\u2013 A eventual cobran\u00e7a de taxas de servi\u00e7os ser\u00e1 feita atrav\u00e9s de uma das funda\u00e7\u00f5es associadas a USP S\u00e3o Carlos, sendo que taxas diferenciadas para as solicita\u00e7\u00f5es provenientes de fora da \u00c1rea acad\u00eamica e externos ao IFSC\/USP podem ser aplicadas.[\/vc_message][vc_message icon_fontawesome=&#8221;fa fa-book&#8221;]<strong>Refer\u00eancias<\/strong><\/p>\n<p>Os trabalhos a serem publicados, bem como teses, disserta\u00e7\u00f5es e monografias dever\u00e3o mencionar o uso do Equipamento Multi-Usu\u00e1rio (EMU\/FAPESP) \/ CIBFar \/ Grupo de Cristalografia \/ IFSC.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-9816\" src=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/fapesp-300x65.jpg\" alt=\"\" width=\"100\" height=\"22\" srcset=\"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/fapesp-300x65.jpg 300w, https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-content\/uploads\/2022\/06\/fapesp.jpg 500w\" sizes=\"auto, (max-width: 100px) 100vw, 100px\" \/>[\/vc_message][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[vc_row rt_row_background_width=&#8221;default&#8221; rt_row_style=&#8221;default-style&#8221; rt_row_borders=&#8221;&#8221; rt_row_paddings=&#8221;true&#8221; rt_bg_effect=&#8221;classic&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;cover&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_bg_video_format=&#8221;self-hosted&#8221;][vc_column rt_wrp_col_paddings=&#8221;false&#8221; rt_border_top=&#8221;&#8221; rt_border_bottom=&#8221;&#8221; rt_border_left=&#8221;&#8221; rt_border_right=&#8221;&#8221; rt_border_top_mobile=&#8221;&#8221; rt_border_bottom_mobile=&#8221;&#8221; rt_border_left_mobile=&#8221;&#8221; rt_border_right_mobile=&#8221;&#8221; rt_bg_image_repeat=&#8221;repeat&#8221; rt_bg_size=&#8221;auto auto&#8221; rt_bg_position=&#8221;right top&#8221; rt_bg_attachment=&#8221;scroll&#8221; rt_padding_left=&#8221;60px&#8221; rt_padding_right=&#8221;60px&#8221;][vc_column_text] MicroScale Thermophoresis (MST) Laborat\u00f3rio de Bioqu\u00edmica no Grupo de Biof\u00edsica e Biologia Estrutural &#8211; IFSC &#8211; USP &#8211; \u00c1rea 2 Comit\u00ea Gestor: Prof. Dr. Glaucius Oliva [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9670","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9670","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/comments?post=9670"}],"version-history":[{"count":16,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9670\/revisions"}],"predecessor-version":[{"id":9959,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9670\/revisions\/9959"}],"wp:attachment":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/media?parent=9670"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}