{"id":9859,"date":"2022-09-21T15:18:06","date_gmt":"2022-09-21T18:18:06","guid":{"rendered":"https:\/\/www.ifsc.usp.br\/multiusuarios\/?page_id=9859"},"modified":"2026-03-24T16:21:50","modified_gmt":"2026-03-24T19:21:50","slug":"cryo-em","status":"publish","type":"page","link":"https:\/\/www.ifsc.usp.br\/multiusuarios\/cryo-em\/","title":{"rendered":"Tundra (TEM) e Aquilos 2 (cryo-FIB SEM)"},"content":{"rendered":"

[vc_row rt_row_background_width=”default” rt_row_style=”default-style” rt_row_borders=”” rt_row_paddings=”true” rt_bg_effect=”classic” rt_bg_image_repeat=”repeat” rt_bg_size=”cover” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_bg_video_format=”self-hosted”][vc_column rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_padding_left=”60px” rt_padding_right=”60px”][vc_column_text]<\/p>\n

Tundra (TEM) e Aquilos 2 (cryo-FIB SEM)<\/h4>\n

Grupo de Cristalografia (GC) \u2013 IFSC \u2013 USP – \u00c1rea 2<\/strong><\/p>\n

Comit\u00ea Gestor\"\"<\/strong><\/header>\n
    \n
  • Prof. Dr. Glaucius Oliva<\/li>\n
  • Prof. Dr. Andre Luis Berteli Ambrosio<\/li>\n<\/ul>\n

    E-mail para contato: oliva@ifsc.usp.br<\/p>\n

    Comit\u00ea de Usu\u00e1rios<\/strong><\/header>\n
      \n
    • Prof. Dr. Richard Charles Garratt<\/li>\n
    • Prof. Dr. Rafael Vict\u00f3rio Carvalho Guido<\/li>\n
    • Facility manager (To be hired)<\/li>\n<\/ul>\n

      [\/vc_column_text][\/vc_column][\/vc_row][vc_row rt_row_background_width=”default” rt_row_style=”default-style” rt_row_borders=”” rt_row_paddings=”true” rt_bg_effect=”classic” rt_bg_image_repeat=”repeat” rt_bg_size=”cover” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_bg_video_format=”self-hosted”][vc_column width=”1\/2″ rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_padding_left=”60px”][vc_message icon_fontawesome=”fa fa-cog”]Equipamentos<\/strong><\/p>\n

      Tundra cryo-TEM <\/b>(Thermo Scientific) \u00e9 um microsc\u00f3pio eletr\u00f4nico de transmiss\u00e3o (TEM) de baixa tens\u00e3o totalmente automatizado, com carregamento e descarregamento r\u00e1pido de amostras, tomada de decis\u00e3o auxiliada por intelig\u00eancia artificial e coleta simplificada de dados para an\u00e1lise de part\u00edculas isoladas (SPA) e avalia\u00e7\u00e3o qualitativa de lamelas celulares (para cryo-ET) ou cristalinas (para micro-ED); Aquilos 2 cryo-FIB SEM <\/b>(Thermo Scientific) \u00e9 um microsc\u00f3pio de duplo feixe \u2013 FIB (feixe focalizado de \u00edons) e SEM (microsc\u00f3pio eletr\u00f4nico de varredura) \u2013 dedicado \u00e0 prepara\u00e7\u00e3o de lamelas el\u00e9tron-transparentes para cryo-ET \u2013 integrado \u00e0 um microsc\u00f3pio de fluoresc\u00eancia (iFLM) para imagem correlativa de el\u00e9trons e luz (CLEM), que permitem a identifica\u00e7\u00e3o e localiza\u00e7\u00e3o de regi\u00f5es subcelulares de interesse. Aquilos 2 tamb\u00e9m permite a prepara\u00e7\u00e3o, por afinamento nanom\u00e9trico, de cristais para micro-ED.[\/vc_message][vc_message icon_fontawesome=”fa fa-user-o”]Plano de Gest\u00e3o de Uso<\/strong><\/p>\n

      Localiza\u00e7\u00e3o<\/u>: Grupo Cristalografia (GC) \u2013 IFSC\/USP, \u00c1rea 2.
      \nProcedimentos para utiliza\u00e7\u00e3o<\/u>: Deve-se preencher, por completo,\u00a0 e submeter o\u00a0formul\u00e1rio espec\u00edfico (em breve) para livia@ifsc.usp.br, incluindo um resumo sucinto do projeto no corpo do e-mail.
      \nCrit\u00e9rios<\/u>: Tundra<\/b>: O usu\u00e1rio deve demonstrar (i) a purifica\u00e7\u00e3o da prote\u00edna de interesse, preferencialmente at\u00e9 homogeneidade, por an\u00e1lise eletrofor\u00e9tica, e (ii) alto grau de homogeneidade populacional, por espalhamento din\u00e2mico de luz, ou equivalente. Aquilos 2<\/b>: O usu\u00e1rio deve demonstrar a viabilidade do sistema celular expressando a amostra de interesse, preferencialmente fusionada \u00e0 cauda fluorescente ou a capacidade de crescimento reprodut\u00edvel de cristais.[\/vc_message][\/vc_column][vc_column width=”1\/2″ rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_padding_right=”60px”][vc_message icon_fontawesome=”fa fa-credit-card-alt”]<\/p>\n

      \n

      Taxas de Servi\u00e7o e Agendamento<\/strong><\/p>\n

      Agendamento<\/u>: As solicita\u00e7\u00f5es ser\u00e3o avaliadas e priorizadas pelo grupo gestor, considerando seu conte\u00fado, relev\u00e2ncia, urg\u00eancia e aplicabilidade, em um fluxo semanal.<\/p>\n

      \"\"<\/p>\n<\/div>\n

      [\/vc_message][vc_message icon_fontawesome=”fa fa-book”]<\/p>\n

      \n

      Refer\u00eancias<\/strong><\/p>\n

      Os trabalhos a serem publicados, incluindo artigos, teses, disserta\u00e7\u00f5es e monografias, dever\u00e3o mencionar o uso do Equipamento Multiusu\u00e1rio e projeto EMU\/FAPESP 2022\/11403-8.<\/p>\n

      \"\"<\/p>\n<\/div>\n

      [\/vc_message][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"

      [vc_row rt_row_background_width=”default” rt_row_style=”default-style” rt_row_borders=”” rt_row_paddings=”true” rt_bg_effect=”classic” rt_bg_image_repeat=”repeat” rt_bg_size=”cover” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_bg_video_format=”self-hosted”][vc_column rt_wrp_col_paddings=”false” rt_border_top=”” rt_border_bottom=”” rt_border_left=”” rt_border_right=”” rt_border_top_mobile=”” rt_border_bottom_mobile=”” rt_border_left_mobile=”” rt_border_right_mobile=”” rt_bg_image_repeat=”repeat” rt_bg_size=”auto auto” rt_bg_position=”right top” rt_bg_attachment=”scroll” rt_padding_left=”60px” rt_padding_right=”60px”][vc_column_text] Tundra (TEM) e Aquilos 2 (cryo-FIB SEM) Grupo de Cristalografia (GC) \u2013 IFSC \u2013 USP – \u00c1rea 2 Comit\u00ea Gestor Prof. Dr. Glaucius Oliva Prof. Dr. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9859","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9859","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/comments?post=9859"}],"version-history":[{"count":6,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9859\/revisions"}],"predecessor-version":[{"id":9966,"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/pages\/9859\/revisions\/9966"}],"wp:attachment":[{"href":"https:\/\/www.ifsc.usp.br\/multiusuarios\/wp-json\/wp\/v2\/media?parent=9859"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}