{"id":183,"date":"2021-08-30T14:39:26","date_gmt":"2021-08-30T17:39:26","guid":{"rendered":"https:\/\/www.ifsc.usp.br\/septins\/?page_id=183"},"modified":"2022-04-19T08:35:24","modified_gmt":"2022-04-19T11:35:24","slug":"research","status":"publish","type":"page","link":"https:\/\/www.ifsc.usp.br\/septins\/research\/","title":{"rendered":"Research"},"content":{"rendered":"<p style=\"text-align: left;\"><span style=\"color: #000000;\">There is still much to be learned about the potential roles of septin structures and structural biology can contribute a lot to this!<\/span><\/p>\n<p><span style=\"color: #000000;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-315 \" src=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1.png\" alt=\"\" width=\"514\" height=\"371\" srcset=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1.png 2229w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-300x217.png 300w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-1024x739.png 1024w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-768x554.png 768w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-1536x1109.png 1536w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-2048x1478.png 2048w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/bio-function-trio-1-624x450.png 624w\" sizes=\"(max-width: 514px) 100vw, 514px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><em><span style=\"color: #000000;\">We use an integrative approach to understand how septins assemble and, ultimately, their biological relevance.<\/span><\/em><\/p>\n<p><span style=\"color: #000000;\">Below, we describe our current research goals, divided into three major themes:<\/span><\/p>\n<hr \/>\n<h1 style=\"text-align: center;\"><span style=\"color: #000000;\">Understanding the mechanism of oligomerization and dynamics of septin complexes<\/span><\/h1>\n<p><span style=\"color: #000000;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-311 \" src=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2.png\" alt=\"\" width=\"470\" height=\"317\" srcset=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2.png 2053w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-300x203.png 300w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-1024x691.png 1024w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-768x518.png 768w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-1536x1037.png 1536w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-2048x1383.png 2048w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/organogram2-624x421.png 624w\" sizes=\"(max-width: 470px) 100vw, 470px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><em><span style=\"color: #000000;\">The arduous road towards the elucidation of a protein structure!<\/span><\/em><\/p>\n<ul>\n<li><span style=\"color: #000000;\">Better understand the flexibility oligomers\/filaments have and how those correlate with the septin ability in recognizing membrane curvature.<\/span><\/li>\n<\/ul>\n<div style=\"width: 625px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-183-1\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2022\/04\/1-s2.0-S002228362100320X-mmc2.mp4?_=1\" \/><a href=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2022\/04\/1-s2.0-S002228362100320X-mmc2.mp4\">https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2022\/04\/1-s2.0-S002228362100320X-mmc2.mp4<\/a><\/video><\/div>\n<p style=\"text-align: center;\"><span style=\"color: #000000;\"><em>Flexibility modes of SEPT2-6-7 oligomers revealed by cryo-EM <\/em><\/span><span style=\"color: #000000;\"><em>(Mendon\u00e7a et al. 2021, reproduced with permission)<\/em><\/span><\/p>\n<ul>\n<li><span style=\"color: #000000;\">Test the hypothesis of the <strong>opening of the NC-interface<\/strong> between two copies of septins of this group <strong>when complexed to GDP <\/strong>(Castro et al. 2020). If correct, then it should be possible to detect a difference in octamer length in the two cases.<\/span><\/li>\n<\/ul>\n<p style=\"line-height: 1.71429; margin-bottom: 1.71429rem;\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-362 aligncenter\" style=\"margin-top: 0.857143rem; margin-bottom: 0.857143rem; color: #000000;\" src=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0-1024x396.png\" alt=\"\" width=\"300\" height=\"116\" srcset=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0-1024x396.png 1024w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0-300x116.png 300w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0-768x297.png 768w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0-624x241.png 624w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/10\/alpha0.png 1101w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<ul>\n<li><span style=\"color: #000000;\">Investigate the <strong>formation of a rare covalent bond<\/strong> <strong>(Cys-Lys, Asn-Lys)<\/strong> between septin subunits (Rosa et al. 2020).<\/span><\/li>\n<li><span style=\"color: #000000;\">Understand the <strong>NC interface between SEPT6 and SEPT7 <\/strong>and its peculiar <strong>heteromeric coiled-coil<\/strong> <span style=\"color: #000000; font-size: 1rem;\"> (Leonardo et al. 2021)<\/span>.\u00a0<\/span><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-435 \" src=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/12\/ImagemCCs.png\" alt=\"\" width=\"430\" height=\"235\" srcset=\"https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/12\/ImagemCCs.png 824w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/12\/ImagemCCs-300x164.png 300w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/12\/ImagemCCs-768x420.png 768w, https:\/\/www.ifsc.usp.br\/septins\/wp-content\/uploads\/2021\/12\/ImagemCCs-624x342.png 624w\" sizes=\"(max-width: 430px) 100vw, 430px\" \/><\/p>\n<ul>\n<li><span style=\"color: #000000;\">Unravel the structural biology of <strong>septins from non-human species<\/strong> where there is currently a dearth of structural information.<\/span><\/li>\n<\/ul>\n<hr \/>\n<h1 style=\"text-align: center;\"><span style=\"color: #000000;\">Septin engineering<\/span><\/h1>\n<ul>\n<li><span style=\"color: #000000;\">Assess<strong> the imp<\/strong><\/span><span style=\"color: #000000;\"><strong>ortance of the C-terminal domain in the selection of partners <\/strong>for septin-septin interaction using coiled-coil chimeras.<\/span><\/li>\n<li><span style=\"color: #000000;\">Study septin interfaces through <strong>non-canonical oligomers<\/strong> composed of a septin mutant (interrupted filaments).<\/span><\/li>\n<li><span style=\"color: #000000;\"><strong>Insert catalytic activity<\/strong> to a non-active septin.\u00a0It is much harder to introduce\/build biological activity compared to simply eliminating it.<\/span><\/li>\n<\/ul>\n<hr \/>\n<h1 style=\"text-align: center;\"><span style=\"color: #000000;\">Interaction with non-septin partners<\/span><\/h1>\n<p><span style=\"color: #000000;\">A huge breakthrough in the septin field will happen once we better comprehend how septins interact with non-septin components and how those regulate the septin cytoskeleton. We just began to characterize these challenging complexes and impressive results are emerging!<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>There is still much to be learned about the potential roles of septin structures and structural biology can contribute a lot to this! We use an integrative approach to understand how septins assemble and, ultimately, their biological relevance. Below, we describe our current research goals, divided into three major themes: Understanding the mechanism of oligomerization [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/full-width.php","meta":{"footnotes":""},"class_list":["post-183","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/pages\/183"}],"collection":[{"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/comments?post=183"}],"version-history":[{"count":60,"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/pages\/183\/revisions"}],"predecessor-version":[{"id":540,"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/pages\/183\/revisions\/540"}],"wp:attachment":[{"href":"https:\/\/www.ifsc.usp.br\/septins\/wp-json\/wp\/v2\/media?parent=183"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}