FAPESP Postdoctoral Position on in situ Cryo-Electron Tomography

São Carlos Institute of Physics (IFSC/USP), São Paulo Research Foundation (FAPESP)

Project Description

This project, financed by the Sao Paulo Research Foundation (FAPESP), aims to study the in situ structure of mitochondrial filamentous enzymes involved in the metabolic adaptation of tumors and their effects on organelle ultrastructure and morphology upon filamentation.

This research will be an extension of Adamoski et al, NSMB 2023 (doi: 10.1038/s41594-023-01118-0), which was recently highlighted in the celebration of NSMB´s 30th anniversary (doi: 10.1038/s41594-024-01248-z). 

Now we focus on more profound questions with cutting-edge methodologies, such as correlative light-electron microscopy (CLEM), cryo-electron tomography in lamella (cryo-ET) and expansion microscopy (ExM), that can be also applied to other proteins in the lab.

Methodological Responsibilities

Sample Preparation: Engage in cell culturing, vitrification, and lamellae preparation in our lab, at the IFSC/USP.

Data Collection and Processing: Perform high-resolution tomographic data collection using top-tier microscopy facilities in Brazil and internationally. Data processing will be done in-house.

Fellowship Details

Duration: Two-year postdoctoral scholarship.

Stipend: R$ 9,047.40 monthly, tax-exempt.

Contingency Fund: Equivalent to 10% of the total fellowship value, allocated for research-related expenses.

Requirements

Educational Background: Doctoral degree in Chemistry, Biochemistry, Molecular Biology, Biophysics, Structural Biology, or related fields.

Technical Skills: Practical experience in cell biology.

Important: Familiarity with FAPESP fellowship requirements (FAPESP Postdoc).

Advantage Criteria

Experience: Demonstrated proficiency in cryo-FIB and cryo-ET, or in single-particle cryo-EM.

Skills: Excellent collaboration abilities. High level of independence and analytical problem-solving. Proficiency in English (written and spoken).

Application Process

Submit the following in Portuguese or English to Andre LB Ambrosio, at andre@ifsc.usp.br, and Sandra MG Dias, at sandra.dias@lnbio.cnpem.br:

• Cover Letter: Max 2 pages, detailing your qualifications, scientific interests, reasons for applying, and alignment with the essential and meriting criteria.
• Curriculum Vitae (CV): Include a publication list.
• Degree Certificate: A copy of your doctoral degree certificate or a document stating the expected date of obtaining your doctorate.
• References: Contact information for two referees willing to provide recommendations.

Application Deadline

Extended to April 20th, 2024 (originally March 31st)

Expected Starting Date (negotiable)

September 1st, 2024

FAPESP Fellowship Opportunity 6788 (POR/EN): link

alba /// March 24, 2024