João Honorato, Katia Oliveira, Celisnolia Leite, Legna Colina-Vegas, Joaquim Nóbrega, Eduardo Castellano, Javier Ellena, Rodrigo Correa, Alzir Batista,
Mononuclear and binuclear RuII/arene/triphenylphosphine complexes with p-substituted benzoic acid derivatives were prepared and characterized. These monocationic complexes of type [Ru(η6-p-cymene)(PPh3)L] (L = benzoic acid (1), p-hydroxybenzoic acid (2), p-nitrobenzoic acid (3) and terephthalic acid (4)) were characterized using various techniques, such as nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and the crystal structure of 1, 3 and 4 were determined by X-ray diffraction analysis. The cytotoxicity of the complexes was evaluated, in vitro, against tumorigenic [MDA-MB-231, MCF-7 (breast), A549 (lung) and DU-145 (prostate)] and non-tumorigenic [MCF-10A (breast), MRC-5 (lung) and PNT-2 (prostate)] cells. The binuclear complex (4) was inactive due to its low solubility. Complexes 1, 2 and 3 showed similar cytotoxicity, however, complex 1 presented better selectivity index against MDA-MB-231 than compounds 2 and 3. Cellular ruthenium absorption was explored by inductively coupled plasma mass spectrometry (ICP-MS) analyzing the whole cells and the culture medium. Complementary studies showed that complex 1 inhibited colony formation, induced morphology changes in cells and promoted cell cycle arrest in the Sub-G1 phase for the MDA-MB-231 cells.
https://doi.org/10.21577/0103-5053.20200076
@article{Honorato_2020, doi = {10.21577/0103-5053.20200076}, url = {https://doi.org/10.21577%2F0103-5053.20200076}, year = 2020, publisher = {Sociedade Brasileira de Quimica ({SBQ})}, author = {Jo{\~{a}}o Honorato and Katia Oliveira and Celisnolia Leite and Legna Colina-Vegas and Joaquim N{\'{o}}brega and Eduardo Castellano and Javier Ellena and Rodrigo Correa and Alzir Batista}, title = {{\textquotedblleft}Half-Sandwich{\textquotedblright}/{RuII} Anticancer Complexes Containing Triphenylphosphine and p-Substituted Benzoic Acids}, journal = {Journal of the Brazilian Chemical Society} }