November 14th to 24th, 2018

Day 1: Introductory Concepts, Wednesday 14th November

  • Room F-210
  • 08:30 - 09:00  Registration/Welcome   Joao Muniz 
    09:00 - 09:30  Overview of the Workshop   (L) E. Krissinel/J. Muniz 
    09:30 - 10:30  Basic Mathematics of Crystallography  (L) A. Buschiazzo 
    10:30 - 11:00  Coffee Break   
    11:00 - 12:00  Theory of x-ray Diffraction  (L) A. Buschiazzo 
    12:00 - 13:30  Lunch   
    13:30 - 14:30  Data Collection - Concepts and Definitions  (L) Nukri Sanishvili 
    14:30 - 15:30  Symmetry and Space groups  (L) A. Lebedev 
    15:30 - 16:00  Coffee Break   
    16:00 - 17:00  CCP4 suite: An Overview  (L) E. Krissinel 
    17:00 - 18:00  Discussion and questions session  All participants 

    Day 2: Data Collection and Processing, Thursday 15th November

    • Anfiteatro Novo
    • 09:00 - 10:00  Data Collection - A Practical Guide  (L) Nukri Sanishvili 
      10:00 - 10:45  Data Processing with Mosflm  (L) A. Leslie 
      10:45 - 11:00  Coffee Break   
      11:00 - 11:45  Data processing with DIALS  (L) J. Parkhurst 
      11:45 - 12:30  Scaling and Merging  (L) A. Leslie 
      12:30 - 13:30  Lunch   
      Lab. Ensino     
      13:30 - 14:15  Student Introduction  All students 
      14:15 - 15:30  Mosflm/Scaling Tutorial  (T) A. Leslie 
      15:30 - 16:00  Coffee Break + Posters installation   
      16:00 - 17:15  DIALS Tutorial  (T) J. Parkhurst 
      17:15 - 20:30  Data Processing Problem Solving 1  (P) All participants 
      20:30 -   Posters installation   

    Day 3: Data Processing & Phasing I: Introduction, Friday 16th November

    • Anfiteatro Novo
    • 09:00 - 09:45  Data Processing with DUI  (L) L. Fuentes-Montero 
      09:45 - 10:30  Twinning and Pseudo-symmetry  (L) A. Lebedev 
      10:30 - 11:00  Coffee Break   
      Lab. Ensino     
      11:00 - 12:30  Dials/DUI Tutorial  (T) L. Fuentes-Montero 
      12:30 - 13:30  Lunch   
      Anfi. Novo     
      13:30 - 14:30  Examples of Data Pathologies  (L) A. Lebedev 
      14:30 - 15:30  Phases and the Phase Problem  (L) Nukri Sanishvili 
      15:30 - 15:45  Coffee Break   
      15:45 - 16:45  Introduction to Molecular Replacement  (L) A. Lebedev 
      16:45 - 17:30  Introduction to Experimental Phasing  (L) R. Read 
      17:30 - 20:00  Posters   
      20:30+  Opening reception (Mosteiro Brewery and Burgers)   

      Day 4: Data Processing & Experimental Phasing, Saturday 17th November

        Anfi. Novo     
        09:00 - 09:45  Introduction to CCP4i2  (L) K. Stevenson 
        09:45 - 10:30  Introduction to Coot  (L) P. Emsley 
        10:30 - 11:00  Coffee Break   
        11:00 - 11:45  Phaser EP & Log Likelihood Gain Maps  (L) R. Read 
        11:45 - 12:30  Expt. Phasing SHELX  (L) I. Uson 
        12:30 - 13:30  Lunch   
        Lab. Ensino     
        13:30 - 14:30  Expt. Phasing with Phaser Tutorial  (T) R. Read 
        14:30 - 15:30  Hands on Problem Solving  (P) All participants 
        15:30 - 16:00  Coffee Break   
        16:00 - 17:00  SHELX Tutorial  (T) I. Uson 
        17:00 - 18:00  Advanced EP with Phaser Tutorial  (T) R. Read 
        18:00 - 20:30  Hands on Problem Solving  (P) All participants 

        Day 5: Free Day, Sunday 18th November (Free Time - tour activity: alternative proposals by the organizers, at the expense of each attendee)



        Day 6: Phasing using the Molecular Replacement Method, Monday 19th November

        • Room F-147
        • 09:00 - 09:45  MR with Phaser  (L) R. Read 
          09:45 - 10:45  MR with Phaser Tutorial  (T) R. Read 
          10:45 - 11:15  Coffee Break   
          11:15 - 12:00  SHELXE for MR  (L) I. Uson 
          12:00 - 12:30  MR Search Models and Assessing the Solution  (L) R. Keegan 
          12:30 - 13:30  Lunch   
          13:30 - 14:15  Phasing with Arcimboldo  (L) I. Uson 
          14:15 - 15:00  Density Modification  (L) P. Bond 
          15:00 - 16:00  ARCIMBOLDO Tutorial  (T) I. Uson 
          16:00 - 16:30  Coffee Break   
          16:30 - 17:00  CCP4MG MR Tutorial  (T) R. Keegan 
          17:00 - 18:00     
          18:00 - 19:00  “Burning Questions” session 1  All participants 
          19:00 - 20:30  Hands on problem solving  (P) All participants 

          Day 7: Refinement & Model Building 1, Tuesday 20th November

          • Anfiteatro Novo
          • 09:00 - 10:00  Advanced MR with Simbad and Ample  (L) A. Simpkin 
            10:00 - 11:00  Refinement with Refmac  (L) Roberto Steiner 
            11:00 - 11:15  Coffee break   
            11:15 - 12:30  Refinement Tutorials  (T) Roberto Steiner
            12:30 - 13:30  Lunch   
            13:30 - 14:30  Building with ARP/wARP  (L) G. Chojnowski 
            14:30 - 15:30  Building with Buccaneer/Nautilus  (L) P. Bond 
            15:30 - 16:00  Coffee break   
            16:00 - 17:00  ARP/wARP Tutorial  (T) G. Chojnowski 
            17:00 - 18:00  Buccaneer/Nautilus Tutorial  (T) P. Bond 
            18:00 - 20:30  Hands on problem solving  (P) All participants 

            Day 8: Refinement & Model Building II, Wednesday 21st November

            • Room F-210
            • 09:00 - 09:45  Advanced Model Building with Coot  (L) P. Emsley 
              09:45 - 10:30  ProSMART & LORESTR - restraints for refinement  (L) Roberto Steiner 
              10:30 - 10:45  Coffee break   
              10:45 - 11:45  Advanced Coot Tutorial  (T) P. Emsley 
              11:45 - 12:30  ProSMART & LORESTR Tutorial  (T) Roberto Steiner 
              12:30 - 13:30  Lunch   
              13:30 - 14:00  Is the SG correct? - Zanuda  (L) A. Lebedev 
              14:00 - 14:45  Ligand Building with ARP/wARP  (L) G. Chojnowski 
              14:45 - 15:30  AceDrg  (L) P. Emsley 
              15:30 - 16:00  Coffee break   
              16:00 - 16:45  ARP/wARP ligand tutorial  (T) G. Chojnowski 
              16:45 - 17:30  AceDrg tutorial  (T) Roberto Steiner 
              17:30 - 20:30  Hands on problem solving  (P) All participants 

              Day 9: Integrative Structural Biology, Thursday 22nd November

              • Anfiteatro Novo
              • 09:00 - 10:00  Cryo-EM - 3D Electron Microscopy  (L) C. Palmer 
                10:00 - 11:00  Refinement CryoEM structures  (L) Roberto Steiner 
                11:00 - 11:30  Coffee break   
                11:30 - 12:30  Fitting models to CryoEM maps  (L) P. Emsley 
                12:30 - 13:30  Lunch   
                13:30 - 16:00  CryoEM Refinement and Building Tutorial  (T) P. Emsley & C. Palmer 
                16:00 - 16:30  Coffee break   
                16:30 - 20:30  Hands on problem solving  (P) All participants 

                Day 10: Validation & Structure Analysis, Friday 23rd November

                • Anfiteatro Novo
                • 09:00 - 09:45  Coot Ligands and Validation  (L) P. Emsley 
                  09:45 - 10:45  Model Quality: Concepts and Statistics  (L) A. Buschiazzo 
                  10:45 - 11:15  Coffee break   
                  11:15 - 11:45  CCP4 Beyond Structure Solution  (L) E. Krissinel 
                  11:45 - 12:30  Analysis of Macromolecular Complexes (PISA)  (L) E. Krissinel 
                  12:30 - 13:30  Lunch   
                  13:30 - 16:00  Hands on problem solving  (P) All participants 
                  16:00 - 16:30  Coffee break   
                  16:30 - 20:30  Hands on problem solving  (P) All participants 

                  Day 11: 3D models from a user’s perspective, Saturday 24th November

                  • Anfiteatro Novo
                  • 09:00 - 10:00  Structure Analysis: What information can be drawn from an atomic model  (L) R. Garratt 
                    10:00 - 11:00  A (very) brief history of Protein Crystallography in Brazil (and beyond)  (L) R. Garratt 
                    11:00 - 11:30  Coffee break   
                    11:30 - 12:30  “Burning Questions” session 2  All Participants 
                    12:30 - 13:30  Lunch   
                    14:00 - 16:30  Students Presentations  All Students 
                    16:30 - 17:00  Final conclusions & Evaluation Form  R. Garratt and J. Muniz 
                    • (L)=Lecture session
                    • (T)=Tutorial session
                    • (P)=Practical session/Problem solving
                    Last update: 24/11/2018 @ 10:25

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